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Anti-NOTCH2 Fc Silent Antibody (FCS-400P) (CAT#: FCS-400P) Datasheet

Product Type
Fc Silent Antibody
Description
The antibody Fc domain is responsible for the function in the antibody, while the Fab domain is responsible for targeting. Therefore, Fc engineering represents the engineering function of antibodies, which are effector functions, such as antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP), and control the serum half-life. Therefore, Fc engineering for immune silencing or further protein engineering is essential for immune cell binding to antibodies.

The Fc-silenced antibody provided by Creative Biolabs contains key point mutations that eliminate the binding of Fc receptors (FcγR, FcR), thereby eliminating the cytotoxic (ADCC) effector function of the antibody.
Species
Human
Clonality
Monoclonal
Isotype
Human IgG2, κ
Conjugation
Unconjugated
Source
Recombinant
Buffer
PBS, pH 7.4
Application
Funcs, In vivo assay
Application Notes
The use of diluent depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution.
Storage Instructions
Product should be stored at -20°C. Repeated freeze and thaw cycles will cause loss of activity. Use product within 24 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.
Target
NOTCH2
Official Name
NOTCH2
Full Name
Notch 2
Background
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Alternative Names
NOTCH2; notch 2; Notch (Drosophila) homolog 2; Notch homolog 2 (Drosophila); neurogenic locus notch homolog protein 2; Notch homolog 2; hN2; AGS2; HJCYS
Gene ID
4853
Uniprot ID
Q04721

All products and services are for Research Use Only. Do Not use in humans.

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