Product Type
Antibody
Description
The monoclonal antibody 16D2 recognizes mouse complement factor C4 (previously known as Gg protein), which is composed of α-, β- and γ chains. The classical pathway of complement and the activation pathway of mannose-binding lectin converge at C4. MASP- 1 and MASP-2 cleave C4 to form C4a and C4b. Subsequently, C4b can be cleaved into C4c and C4d by other serum enzymes. The monoclonal antibody 16D2 reacts with intact C4, C4b and C4d.
C4 is an acute phase protein produced by hepatocytes, monocytes and intestinal epithelial cells. It can be used as a marker for activating the classical complement pathway in experimental animals. Recent studies have shown that there is an association between transplant rejection and C4d deposition in a mouse model of heart transplantation.
Disease
Infectious diseases
Size
100 µg
Immunogen
Thymocytes decorated with Thy-1 antibody and complement components
Species
Mouse
Clonality
Monoclonal
Clone Number
16D2
Buffer
PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Application
Immuno assays, Immuno fluorescence, Immuno precipitation, Western blot
Application Notes
For immunoassays, immunofluorescence, immunoprecipitation and western blotting, the diluent to be used depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Alternative Names
Complement component 4
