Product Type
Antibody
Description
The monoclonal antibody 3D5 recognizes complement decline acceleration factor (DAF), also known as CD55. Cells express several proteins on their surface that protect against complement attack, namely C receptor I (CR1), decay acceleration factor, membrane cofactor protein (MCP). CR1, CD55 and MCP regulate the activation pathway of complement by accelerating the degradation of C3 and C5 convertases (CR1, CD55) or acting as cofactors of serine protease factor I. The cleavage of this factor irreversibly inactivates C3b ( CR1, MCP).
Mouse CD55 is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit the formation and half-life of C3 convertase. CD55 is widely distributed in cells in contact with serum, including hematopoietic and non-hematopoietic cells. Although CD55 has no important role in controlling hemolysis of red blood cells, it plays an important role in regulating the deposition of C3 on nucleated cells.
CD55 and other complement regulators can protect one's own cells from damage mediated by autologous complement. CD55 and CD46 together prevent the deposition of autologous C3, while CD59 inhibits this pathway at a key endpoint.
Disease
Autoimmunity, Infectious diseases
Size
100 µg
Immunogen
NRK cells expressing transmembrane-anchored mouse DAF
Species
Mouse
Clonality
Monoclonal
Clone Number
3D5
Conjugation
FITC
Buffer
PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Application
Flow cytometry, Immuno assays, Paraffin sections, Western blot
Application Notes
The diluent used depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Alternative Names
Complement decay-accelerating factor; GPI-anchored; DAF-GPI
