Product Type
Fc Silent Antibody
Description
The Fc-mediated effector functions of antibodies include antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), and complement-dependent cytotoxicity (CDC), and have been shown to be crucial for the therapeutic efficacy of most clinically approved antibodies. However, engaging immune effectors through FcγR and complement interactions may be detrimental to certain mAb mechanisms of action and so Fc-null or -silenced antibodies may be desired.
Creative Biolabs uses site-directed mutagenesis technology to generate Fc-silenced antibody with reduced effector functions. The mutated Fc domains eliminate the binding of Fc receptors (FcγR, FcR), thereby leading to a significant reduction in ADCC and CDC in comparison to WT IgG.
Immunogen
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTα expressed in E. coli - the hybridoma clone was selected for its ability to bind LTα3 by ELISA. The CDRs of the a...
Species
Human
Cross reactivity
Human
Clonality
Monoclonal
Clone Number
3F12.2D3
Conjugation
Unconjugated
Buffer
PBS only
Application
N, EL, FC, SPR
Storage Instructions
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Alternative Names
LTA; LT; TNFB; TNFSF1; TNLG1E
