Connect with Us at the Upcoming Events

With in-person events back again, Creative Biolabs is excited to greet you at the upcoming international conferences. Meet our team at 2022 BIO International Convention in June (booth #1754) and at the 13th Annual World ADC San Diego in September (booth number to be updated) for expert consultation on your drug discovery. Shoot an email to arrange an in-person meeting!

Book a Meeting

Anti-Human MASP-3 Monoclonal Antibody (clone 38:12-3) -100 µg (CAT#: CB-P186-AB) Datasheet

Product Type
Antibody
Description
Monoclonal antibody 38:12-3 recognizes MASP-3, a 94 kDa mannan-binding lectin (MBL)-related serine protease. Three pathways of complement activation have been reported: antibody-dependent classical pathway, antibody-independent alternative pathway and lectin pathway. The activation of each pathway involves the formation of a serine protease complex, which leads to the activation of the central complement component C3. In the lectin pathway, mannose-binding lectin (MBL)-related serine protease (MASP) forms a complex with oligolectin molecules, and the latter is involved in pattern recognition. After the recognition molecule binds to carbohydrates on the surface of the microorganism, MASP is converted to its active form and initiates complement activation. Six members of the MASP-like serine protease family have been reported: MASP-1, MASP-2, MASP-3, Map19, C1r and C1s. These products show the same domain organization, even though the total amino acid sequence identity is only about 40%. Each MASP type forms a homodimer and binds to MBL and fibrin respectively in a Ca2+ dependent manner. The four MBL-related proteins are produced by only two genes. The MASP-1/3 gene encodes MASP-1 and MASP-3, while the MASP-2/Map19 gene produces MASP-2 and non-enzymatic MASP-2 MAP19. MASP-1 preferentially associates with the oligomer MBL (called MBL-1), while MASP-2 and MASP-3 mainly associate with the larger MBL oligomers (MBL II and MBL III). MASP-3 is produced by alternative splicing of the MASP-1/3 gene. The MASP-3 transcript is composed of the A chain shared by MASP-1 and MASP-3 and the unique B chain of MASP-3. The biological function of MASP-3 remains unclear. However, it was found that MASP-3 down-regulated the C4 and C2 lytic activity of MASP-2. MASP-3 inhibits MASP-2 by competitively inhibiting the binding of MASP-2 and MBL and inhibiting the activation of MASP-2 associated with MBL. Monoclonal antibody 38:12-3 cannot recognize MASP-1.
Disease
Autoimmunity, Infectious diseases, Nephrology
Size
100 µg
Species
Human
Host
Rat
Clonality
Monoclonal
Isotype
Rat IgG2a
Clone Number
38:12-3
Buffer
PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Application
Immuno assays, Western blot
Application Notes
The diluent used depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Target
MASP-3
Alternative Names
MASP3

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK