Description
Anti-Chemokine receptor CXCR4 Antibody, Non-Fucosylated (BioBet-761ZP) is a humanized monoclonal IgG antibody against Chemokine receptor CXCR4. This product is an ADCC enhanced antibody produced by our Afuco™ platform.
Antibody Indication
Cancer; Hematopoietic stem cell mobilization
Classification
Therapeutic antibody; biobetter
Cooperation Seeking
Creative Biolabs is interested in collaborating with potential partners (include but not limit to major pharma or biotech firms) to further co-develop ADCC-enhanced Chemokine receptor CXCR4 antibody. For commercial partners interested in our ADCC-enhanced therapeutic antibodies, Creative Biolabs welcomes collaboration. Here are two ways for your choice, and please contact us for more details.
1) Collaborate with us and co-develop the programs from discovery phase to IND enabling. Costs will be shared.
2) Become a licensed candidate of our programs.
Looking forward to cooperating with you in the near future.
Official Name
CXCR4
Full Name
chemokine (C-X-C motif) receptor 4
Background
This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Alternative Names
CXCR4; chemokine (C-X-C motif) receptor 4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E; C-X-C chemokine receptor type 4; fusin; CD184 antigen; SDF-1 receptor; LPS-associated protein 3; neuropeptide Y receptor Y3; seven transmembrane helix receptor; stromal cell-derived factor 1 receptor; lipopolysaccharide-associated protein 3; seven-transmembrane-segment receptor, spleen; leukocyte-derived seven transmembrane domain receptor
Gene ID
7852
UniProt ID
Q8WTT0
Cellular Localization
Plasma membrane, Endosome, Lysosome
Involvement in Disease
Diseases associated with CXCR4 include Whim Syndrome and Macroglobulinemia.
Related Pathways
Its related pathways are Neuroscience and Signaling by GPCR.
Function
The receptor of C-X-C chemokine CXCL12/SDF-1 transmits signals by increasing intracellular calcium levels and enhancing MAPK1/MAPK3 activation. Participate in the AKT signal cascade (PubMed: 24912431). Participate in regulating cell migration, such as during wound healing. As a receptor for extracellular ubiquitin; leading to increased intracellular calcium ions and decreased cell cAMP levels. Combined with bacterial lipopolysaccharide (LPS) et mediates lipopolysaccharide-induced inflammation, including monocyte secretion of TNF. Participate in the formation of hematopoiesis and ventricular septum. It may also play an important role in the vascularization of the gastrointestinal tract by regulating the vascular branching and/or remodeling process of endothelial cells. Participate in cerebellar development. In the central nervous system, it can mediate the survival of hippocampal neurons (through similarity). (Microbial infection) As a co-receptor for human immunodeficiency virus-1/HIV-1 X4 isolates (CD4 is the main receptor), and as the main receptor for some HIV-2 isolates. Promote env-mediated virus fusion.
Field of research
Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody
Post-translational modifications
Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation. Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S. Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization. Tyr-7 and Tyr-12 are sulfated in a sequential manner after Tyr-21 is almost fully sulfated, with the binding affinity for CXCL12/SDF-1alpha increasing with the number of sulfotyrosines present. Sulfotyrosines Tyr-7 and Tyr-12 occupy clefts on opposing CXCL12 subunits, thus bridging the CXCL12 dimer interface and promoting CXCL12 dimerization. O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity.
Biologic Classification
Protein Based Therapies
Monoclonal antibody (mAb)
Antibody Isotype
IgG
Antibody Clone
ALX-0651
Description
ALX-0651 is the first sdAb in clinical trials targeting a GPCR, for use in stem cell mobilisation. CXCR4 plays a key regulatory role in hematopoietic stem cell trafficking.
Antibody Indication
Cancer; Hematopoietic stem cell mobilization
Cancer; Hematopoietic stem cell mobilization
