Product Type
Fc Silent Antibody
Description
The Fc-mediated effector functions of antibodies include antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), and complement-dependent cytotoxicity (CDC), and have been shown to be crucial for the therapeutic efficacy of most clinically approved antibodies. However, engaging immune effectors through FcγR and complement interactions may be detrimental to certain mAb mechanisms of action and so Fc-null or -silenced antibodies may be desired.
Creative Biolabs uses site-directed mutagenesis technology to generate Fc-silenced antibody with reduced effector functions. The mutated Fc domains eliminate the binding of Fc receptors (FcγR, FcR), thereby leading to a significant reduction in ADCC and CDC in comparison to WT IgG.
Immunogen
This antibody was generated by immunizing female BALB/c mice with a fusion protein containing extracellular domain of human CD40 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody.
Species
Human
Cross reactivity
Human
Clonality
Monoclonal
Clone Number
chi220
Conjugation
Unconjugated
Source
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Buffer
PBS only
Application
BL, Therapeutic
Storage Instructions
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Official Name
CD40LG
Alternative Names
CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand
