Description
Creative Biolabs' newly designed ADP assay kit provides a convenient fluorescence assay method that can determine ADP levels even in the presence of ATP. In the assay, ADP is converted to ATP and pyruvate. Then quantify the generated pyruvate by fluorescence method (Ex / Em = 530 / 590nm). The determination method is simple, sensitive, stable, high-throughput adaptability, and can detect as low as 0.1 ADP in biological samples.
Features
•Safety. Non-radioactive determination.
•Sensitive and accurate. It can be quantified as low as 0.1 ADP.
•Similar and convenient. Type determination of "mixed cultivation measures". No washing and reagent transfer steps are involved.
•Rugged and suitable for HTS: It can be easily automated on the HTS liquid handling system, processing thousands of samples every day.
Applications
For the quantitative determination of ADP in cells and other biological samples.
Size
100 tests
Detection Method
FL530/590nm
Detection Limit
0.1 uM
Sample
Cells and other biological samples
Protocol Length
30 min
Components
Reagent A: 6 mL Enzyme: 120 uL 10% TCA: 6 mL Reagent B: 6 mL Standard: 100 uL Neutralizer: 1.5 mL
Requires
Pipetting devices and accessories (e.g. multi-channel pipettor), black flat-bottom 96-well plates, centrifuge tubes and plate reader.
Shipping
Gel Pack
Storage
Store all reagents at -20°C
Shelf Life
6 months
Research Use
For Research Use Only! Not For Use in Humans.
