Rat FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Strep II Tag (SPR verified) [Biotinylated] (CAT#: FC-P016-ZP) Datasheet

Product Type
Protein
Description
Biotinylated Rat FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.
Species
Rat
Conjugation
Biotin
Construction
It contains AA Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P13599-1 (FCGRT) & P07151-1 (B2M)).
Expression
HEK293
N-Terminal
Predicted N-terminus: Ala 23 (FCGRT) & Ile 21 (B2M)
Tag
His Tag & Strep II Tag
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Buffer
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Target
FcRn (FCGRT & B2M)
Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
Alternative Names
FcRn, FCGRT & B2M

Figure 1 Molecular Characterization

Figure 2 SDS-PAGE

Figure 3 Bioactivity-BLI

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK