Mouse FcRn / FCGRT&B2M Heterodimer Protein, Avi Tag,His Tag&Twin-Strep Tag (BLI verified) [Biotinylated] (CAT#: FC-P012-ZP) Datasheet

Product Type
Protein
Description
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 15 kDa (B2M) respectively due to glycosylation.
Species
Mouse
Conjugation
Biotin
Construction
It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1 (FCGRT) & P01887-1 (B2M)).
Expression
HEK293
N-Terminal
Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)
Tag
His Tag
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Buffer
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Target
FcRn (FCGRT & B2M)
Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
Alternative Names
FcRn, FCGRT & B2M

Figure 1 Molecular Characterization

Figure 2 SDS-PAGE

Figure 3 Bioactivity-BLI

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