Human Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified) (CAT#: FC-P035-ZP) Datasheet

Product Type
Protein
Description
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.4 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Species
Human
Construction
It contains AA Ala 46 - Pro 217 (Accession # P31994).
Expression
HEK293
N-Terminal
Predicted N-terminus: Ala 46
Tag
His Tag
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Buffer
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Target
Fc gamma RIIB / CD32b
Background
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
Alternative Names
FCGR2B, CD32b, CD32, FCG2, IGFR2, CDw32

Figure 1 Molecular Characterization

Figure 2 SDS-PAGE

Figure 3 Bioactivity-SPR

Figure 4 Bioactivity-BLI

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