Human Fc gamma RIIA / CD32a (R167) Protein, His Tag (BLI verified) (CAT#: FC-P029-ZP) Datasheet

Product Type
Protein
Description
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Species
Human
Construction
It contains AA Ala 36 - Ile 218 (Accession # P12318-1 (R167)).
Expression
HEK293
N-Terminal
Predicted N-terminus: Ala 36
Tag
His Tag
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Buffer
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Target
Fc gamma RIIA / CD32a
Background
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
Alternative Names
CD32a, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2

Figure 1 Molecular Characterization

Figure 2 SDS-PAGE

Figure 3 Bioactivity-SPR

Figure 4 Bioactivity-BLI

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