Human Fc gamma RI / CD64 Protein, His,Avi Tag (MALS verified) [Biotinylated] (CAT#: FC-P025-ZP) Datasheet

Product Type
Protein
Description
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 34.2 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Species
Human
Conjugation
Biotin
Construction
It contains AA Gln 16 - Pro 288 (Accession # P12314-1).
Expression
HEK293
N-Terminal
Predicted N-terminus: Gln 16
Tag
His Tag
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Buffer
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Target
Fc gamma RI / CD64
Background
Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFN款 and G-CSF can induce CD64 expression on these cells.
Alternative Names
FCGR1A, FCG1, FCGR1, IGFR1, CD64, CD64A, FCRI

Figure 1 Molecular Characterization

Figure 2 SDS-PAGE

Figure 3 Bioactivity-SPR

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