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Human C5a des Arg Protein, Recombinant - 50 µg (CAT#: CB-P286-AB) Datasheet

Product Type
Protein
Description
Complement fragment C5a is a 74-residue glycopolypeptide, which is produced by proteolytic cleavage of complement factor C5 during complement activation. C5a is an effective chemotactic and anaphylactic toxin that can act on all types of white blood cells and many other cell types, including endothelial cells, smooth muscle, kidney, liver and nerve cells. In addition to its pro-inflammatory effects, C5a has also been shown to protect cells from toxicity and stimulate the proliferation of neurons and hepatocytes, which indicates that C5a has a broader role in homeostasis. C5a is rapidly deprived of arginine by serum carboxypeptidase N and becomes the less potent derivative C5a desArg, which is the first step to inactivate anaphylactoxin activity. The C5a desArg form has a different spectrum of biological activity to intact C5a. Recombinant human C5a desArg has a His tag and has the following amino acid sequence: MRGSHHHHHHHHGSDYDIPTTENLYFQGGSTLQKKIEEIAAKYKHSVVKKCCYDGACVNNDETCEQ RAARISL​​GPRCIKAFTECCVVASQLRANISHKDMQLG ((His-tag is underlined), bold sequence is underlined
Disease
Infectious diseases, Nephrology
Size
50 µg
Species
Human
Buffer
Lyophilized product in 0.005% Tween 80, 2mM reduced L-glutathion, 0.2mM oxidized L-glutathion and 0.1M Tris-Cl buffer, pH 8.0, containing at least 50 μg human C5a desArg. The exact amount is indicated on the label. Reconstitute the vial by pipetting 0.5 ml distilled or de-ionised water (Caution: vial is under vacuum).
Application
Functional studies, Immuno assays, Western blot
Application Notes
For dilution, use protein-stabilized phosphate buffered saline, pH 7.4.
Storage Instructions
Product should be stored at -80°C. Repeated freeze and thaw cycles will cause loss of activity. Use C5 protein within 39 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.
Target
C5a des Arg
Alternative Names
C5; C5D; C5a; C5b; CPAMD4; ECLZB; complement component 5; complement C5

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