Human C5 Protein, Natural - 200 µg (CAT#: CB-P271-AB) Datasheet

Product Type
Protein
Description
Complement C5 has been isolated from human blood. C5 (molecular weight is about 190kDa) is a key component of the complement system. This system plays an important role in both innate and adaptive immune responses, and can produce inflammatory and protective responses to pathogen attacks before the adaptive response occurs. The complement system consists of a complex family of proteins and receptors, which are found in the circulatory system, tissues and other body fluids. Excessive activation and uncontrolled regulation of the complement system involve many diseases, such as asthma, lupus erythematosus, glomerulonephritis, various forms of arthritis, autoimmune heart disease, multiple sclerosis, inflammatory bowel disease, paroxysmal Nocturnal hemoglobinuria, atypical hemolytic uremic syndrome and ischemia-reperfusion injury and transplant rejection. Three main pathways within the complement system have been described, namely the classical pathway, the alternative pathway and the lectin pathway, which are activated by different stimuli. The classical pathway is triggered by immune complexes. Through the lectin pathway of surface-bound lectins; and protect AP through all unprotected surfaces. All three pathways converge on complement C3. The cleavage of C3 induces the cleavage of C5, which in turn leads to the production of membrane attack complex (MAC). This protein complex is the ultimate common effector responsible for lysing susceptible microorganisms or destroying cells. Mutations in the C5 gene can cause a deficiency of complement component 5. This disease is characterized by repeated bacterial infections.
Disease
Autoimmunity
Size
200 µg
Species
Human
Buffer
PBS, pH 7.4
Application
Functional studies, Western blot
Application Notes
The use of diluent depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution.
W: Reduce sample handling and use SDS-Page. The band size is approximately 150 kDa.
FS: Complement activation after incubation with CVF is tested by lysis of sheep red blood cells.
Storage Instructions
Product should be stored at -80°C. Repeated freeze and thaw cycles will cause loss of activity. Use C5 protein within 24 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.
Target
C5
Alternative Names
C5; complement C5

All products and services are for Research Use Only. Do Not use in humans.

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