Human C2 Protein, Natural - 50 µg (CAT#: CB-P283-AB) Datasheet

Product Type
Protein
Description
C2 is critical for the activation of the classical and lectin pathways of complement. It forms the proteolytic subunits of C3 and C5 convertases in both pathways. The initiation of each pathway produces a proteolytic enzyme complex that binds to the target surface (C1q / C1r / C1s in the classical pathway, MBL / Ficolin / MASP in the lectin pathway). Both C1 and MASP in these complexes activate C4 and C2. They cleaved the peptide bond in C4 and deposited C4b on the surface. They also cut C2 into two fragments. The larger fragment (C2a) binds to C4b and forms the C3/C5 convertase complex C4b, C2a. C2 and B factors are the two most heat-sensitive complement components. Incubate at 56°C for 6 minutes, both may be largely inactivated. Care must be taken not to inactivate many other heat-sensitive complement components. It should be noted that heat inactivation will only reduce the concentration of active C2, and will not completely inactivate or remove C2 protein.
Disease
Infectious diseases, Nephrology
Size
50 µg
Species
Human
Buffer
0.1 ml of approximately 0.5 mg/ml purified natural human C2 isolated from healthy blood donors, in Phosphate buffered saline, pH 6.0. The blood donors have been tested and found negative for various viruses (see table: Human blood test results). The exact protein concentration and functional activity is indicated on the label.
Application
Functional studies
Application Notes
For functional studies, the in vitro dilution must be optimized in the user's experimental setting.
Storage Instructions
Product should be stored at -80°C. Repeated freeze and thaw cycles will cause loss of activity. Use C5 protein within 36 hours after thawing and keep on ice. Remainder amounts should be aliquoted and immediately re-freezed for future use. Aliquots should never be thawed more than once. Under recommended storage conditions, product is stable for at least one year.
Target
C2
Alternative Names
Complement component C2

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