Feline FcRn / FCGRT&B2M Heterodimer Protein, His,Avi Tag (SPR verified) [Biotinylated] (CAT#: FC-P018-ZP) Datasheet

Product Type
Protein
Description
Biotinylated Feline FCGRT&B2M Heterodimer Protein, His,Avitag (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.0 kDa (FCGRT) and 11.5 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag. The reducing (R) protein migrates as 36-40 kDa (FCGRT) and 18-20 kDa (B2M) respectively due to glycosylation.
Species
Feline
Conjugation
Biotin
Construction
It contains AA Ala 24 - Ser 297 (FCGRT) & Val 21 - Met 118 (B2M) (Accession # W5VRL5-1 (FCGRT) & Q5MGS7-1 (B2M)).
Expression
HEK293
N-Terminal
Predicted N-terminus: Ala 24 (FCGRT) & Val 21 (B2M)
Tag
His Tag
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Buffer
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Target
FcRn (FCGRT & B2M)
Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
Alternative Names
FcRn, FCGRT & B2M

Figure 1 Molecular Characterization

Figure 2 SDS-PAGE

Figure 3 Bioactivity-BLI

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