Fc Receptor Proteins

The efficacy of therapeutic antibodies depends not only on the Fab fragment and its binding activity to the target antigen, but also on the Fc fragment and its interaction with key Fc receptors. The binding affinity between the Fc fragment and FcRn (FCGRT&B2M) will predict the half-life of the antibody, and the binding affinity between the Fc fragment and FcγRIIIa (CD16a) will affect the antibody's ability to trigger ADCC (antibody-dependent cell-mediated cytotoxicity). Therefore, candidates must be tested against a set of receptors during the antibody engineering process.

Creative Biolabs provides a complete collection of recombinant Fc receptor proteins, including their common variants, to help you speed up the development of mAbs.

Key Features

High Purity

In order to meet the high purity requirements of the pharmaceutical industry, our production team performed SDS-PAGE and HPLC analysis on the Fc receptor for quality control. It is worth noting that a monomeric Fc receptor that represents its natural state must be used. The formation of oligomers during the production process may result in artificially enhanced binding interactions due to affinity effects. To avoid this situation, we established strict internal control standards for monomer purity through HPLC testing. Only those batches that meet all purity requirements are released.

The purity of human FcRn (FCM-H5286) was greater than 95% as determined in a HPLC analysis. Fig.1 The purity of human FcRn (FCM-H5286) was greater than 95% as determined in a HPLC analysis.

Biotinylated Proteins Available

The use of biotin markers can make your assay development easier. We provide various ready-made biotinylated Fc receptors. These proteins are produced using our internally developed labeling technology, which provides high biological activity and minimal batch-to-batch variation.

Biotinylated Human FcRn / FCGRT & B2M, Avi Tag coupled to SA coated sensor chip can bind Herceptin with an affinity constant of 2.24 μM as determined in a SPR assay Fig.2 Biotinylated Human FcRn / FCGRT & B2M, Avi Tag coupled to SA coated sensor chip can bind Herceptin with an affinity constant of 2.24 μM as determined in a SPR assay (Biacore 2000).

Bioactivity Validated by SPR&BLI

The study of Fc interaction is usually done by SPR, because traditional ELISA is not suitable for low-affinity receptors such as CD16 and CD32. In order to ensure that our Fc receptors can provide the expected performance, our QC team uses the Biacore platform to conduct reference antibody tests on our products. In addition, in order to meet more applications in antibody drug development, our QC team also uses the ForteBio platform to test our products against reference antibodies.

Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag on CM5 Chip via Anti-His antibody, can bind Herceptin with an affinity constant of 0.261 μM as determined in a SPR assay Fig. 3 Immobilized Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag on CM5 Chip via Anti-His antibody, can bind Herceptin with an affinity constant of 0.261 μM as determined in a SPR assay (Biacore T200).

Selection Guides for Protein Conjugates

FcRn / FCGRT & B2M
FC-P001-ZP Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (BLI verified)
FC-P002-ZP Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His Tag (BLI verified)
FC-P003-ZP Cynomolgus / Rhesus macaque FcRn / FCGRT&B2M Heterodimer Protein, His,Avi Tag (SPR verified) [Biotinylated]
FC-P004-ZP Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avi Tag&Strep II Tag (BLI verified) [Biotinylated]
FC-P005-ZP Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified)
FC-P006-ZP Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, ultra sensitivity (primary amine labeling) (SPR verified) [Biotinylated]
FC-P007-ZP Human FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Strep II Tag (SPR & BLI verified) [Biotinylated]
FC-P008-ZP Human FcRn / FCGRT&B2M Heterodimer Protein, His,Avi Tag (SPR verified) [Biotinylated]
FC-P009-ZP Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified)
FC-P010-ZP Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (BLI verified)
FC-P011-ZP Mouse FcRn / FCGRT&B2M Heterodimer Protein, His,Avi Tag (BLI verified) [Biotinylated]
FC-P012-ZP Mouse FcRn / FCGRT&B2M Heterodimer Protein, Avi Tag,His Tag&Twin-Strep Tag (BLI verified) [Biotinylated]
FC-P013-ZP Porcine FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified)
FC-P014-ZP Rat FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified)
FC-P015-ZP Rabbit FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (SPR verified)
FC-P016-ZP Rat FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Strep II Tag (SPR verified) [Biotinylated]
FC-P017-ZP Bovine FcRn / FCGRT&B2M Heterodimer Protein, His,Avi Tag (SPR verified) [Biotinylated]
FC-P018-ZP Feline FcRn / FCGRT&B2M Heterodimer Protein, His,Avi Tag (SPR verified) [Biotinylated]
FC-P019-ZP Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified)
CD23
FC-P020-ZP Human CD23 / Fc epsilon RII Protein, His Tag
FC-P021-ZP Human CD23 / Fc epsilon RII Protein, His,Avi Tag [Biotinylated]
Fc gamma RI / CD64
FC-P022-ZP Mouse Fc gamma RI / CD64 Protein, His Tag (BLI verified)
FC-P023-ZP Cynomolgus Fc gamma RI / CD64 Protein, His,Avi Tag (BLI verified) [Biotinylated]
FC-P024-ZP Human Fc gamma RI / CD64 Protein, His Tag (MALS verified)
FC-P025-ZP Human Fc gamma RI / CD64 Protein, His,Avi Tag (MALS verified) [Biotinylated]
Fc gamma RIIA / CD32a
FC-P026-ZP Human Fc gamma RIIA / CD32a (H167) Protein, His Tag (SPR & BLI verified)
FC-P027-ZP Cynomolgus Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI & HPLC verified)
FC-P028-ZP Cynomolgus Fc gamma RIIA / CD32a Protein, His,Avi Tag (SPR & BLI verified) [Biotinylated]
FC-P029-ZP Human Fc gamma RIIA / CD32a (R167) Protein, His Tag (BLI verified)
FC-P030-ZP Human Fc gamma RIIA / CD32a (H167) Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
FC-P031-ZP Human Fc gamma RIIA / CD32a (R167) Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
FC-P032-ZP Rhesus macaque Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI verified)
Fc gamma RIIB / CD32b
FC-P033-ZP Cynomolgus Fc gamma RIIB / CD32b Protein, His Tag
FC-P034-ZP Cynomolgus Fc gamma RIIB / CD32b Protein, His,Avi Tag (SPR & BLI verified) [Biotinylated]
FC-P035-ZP Human Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified)
FC-P036-ZP Human Fc gamma RIIB / CD32b Protein, SUMO,His Tag
FC-P037-ZP Human Fc gamma RIIB / CD32b Protein, HSA,His Tag
FC-P038-ZP Human Fc gamma RIIB / CD32b Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
FC-P039-ZP Mouse Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified)
FC-P040-ZP Mouse Fc gamma RIIB / CD32b Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
Fc gamma RIII / CD16
FC-P041-ZP Mouse Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI verified)
FC-P042-ZP Cynomolgus Fc gamma RIII / CD16 Protein, His Tag (BLI verified)
FC-P043-ZP Cynomolgus Fc gamma RIII / CD16 Protein, His,Avi Tag (BLI verified) [Biotinylated]
FC-P044-ZP Rhesus macaque Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI verified)
Fc gamma RIIIA / CD16a
FC-P045-ZP Human Fc gamma RIIIA / CD16a (V176) Protein, His Tag (SPR & BLI verified)
FC-P046-ZP Human Fc gamma RIIIA / CD16a (F176) Protein, His Tag (SPR & BLI verified)
FC-P047-ZP Human Fc gamma RIIIA / CD16a (V176) Protein, SUMO,His Tag
FC-P048-ZP Human Fc gamma RIIIA / CD16a (V176) Protein, HSA,His Tag
FC-P049-ZP Human Fc gamma RIIIA / CD16a (F176) Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
FC-P050-ZP Human Fc gamma RIIIA / CD16a (V176) Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
Fc gamma RIIIB / CD16b
FC-P051-ZP Human Fc gamma RIIIB / CD16b (NA1) Protein, His Tag (SPR & BLI verified)
FC-P052-ZP Human Fc gamma RIIIB / CD16b (NA1) Protein, His,Avi Tag (SPR & BLI verified) [Biotinylated]
FC-P053-ZP Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI verified)
FC-P054-ZP Human Fc gamma RIIIB / CD16b (NA2) Protein, His,Avi Tag (SPR & BLI verified) [Biotinylated]
Fc gamma RIV / CD16-2
FC-P055-ZP Mouse Fc gamma RIV / CD16-2 Protein, His Tag
FC-P056-ZP Mouse Fc gamma RIV / CD16-2 Protein, His,Avi Tag (BLI verified) [Biotinylated]
Fc Protein
CB-P445-K Recombinant Human Fc receptor-like protein 1 (FCRL1)
CB-P446-K Recombinant Human Fc Protein, IgG1
CB-P447-K Recombinant Human Fc Protein, IgG2
CB-P448-K Recombinant Human Fc Protein, IgG4
CB-P449-K Recombinant Mouse Fc Protein, IgG1

Please browse the options below to take a deeper look at the tools for your research needs.

RESOURCES

Creative Biolabs provides luciferase-based ADCC assay. This Jurkat cell based assay is pioneered by Creative Biolabs, and the methodology is very well accepted by the field. See attached ADCC Reporter Assay Protocol for further details. 

All products and services are for Research Use Only. Do Not use in humans.

CASE STUDY

ADCC Assay WT vs AfucoTM Mabs  Visit

Resource

Antibody Fc Engineering: Towards Better Therapeutics  Visit

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *