Cell Fractionation Kit (CAT#: CB-P011-K) Datasheet

The cell fractionation system is designed to reproducibly extract four subcellular protein fractions (cytosol, nucleus, membrane/particle and cytoskeleton fraction) from a single mammalian sample. The method is quick and simple, and only takes 2 hours without ultracentrifugation. All four protein fractions obtained are suitable for many downstream applications, such as 1-D or 2-D gels, enzyme activity determination, gel shift determination and Western blotting.

•The procedure is simple, less than 1 hour
•Fast and convenient
•The kit can be used to continuously prepare four different protein components from one sample, including cytoplasm/particle/cytoskeleton/nuclear components.

1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.