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Cell Cytotoxicity Assay Kit (Colorimetric) (CAT#: CB-P056-K) Datasheet

Description
Monitoring the cytotoxicity of cells is one of the most important tasks for studying cell functions. A variety of parameters can be used. Creative Biolabs' uses proprietary water-soluble dyes that can change the absorption spectrum of cells after reduction. The change in absorption rate is directly proportional to the number of living cells. Its high sensitivity, non-radioactive and no-clean features make the kit suitable for high-throughput screening of cell proliferation or cytotoxicity against multiple compounds.

Compared with tetrazole-based colorimetric assays (such as MTT and XTT), the kit does not require pre-mixing of components and has higher sensitivity. It contains enough reagents for 1000 measurements. The kit components are very stable and have minimal cytotoxicity. Therefore, if necessary, the incubation time can be extended (for example, 24-48 hours).

The kit is sturdy and easy to use. It can be easily adapted to various instrument platforms. The assay can be performed in a convenient 96-well or 384-well microtiter plate format.
Detection Method
Colorimetric
Sample
Adherent cells, Suspension cells
Species Reactivity
Mammals, Other species
Components
Assay Solution:Component A
Requires
Microplate reader
Shipping
Gel Pack
Storage
-20°C
Shelf Life
24 months from date of purchase, if stored properly.
Research Use
For Research Use Only! Not For Use in Humans.

Functional Studies

CHO-K1 cell number response measurement. Inoculate 0 to 10,000 cells/well/100 µL of CHO-K1 cells in a 96-well black wall/clear bottom plate overnight. Incubate the cells with 20 µL/well of the assay solution at 37 degrees for 3 hours. The absorbance intensity was measured at 570nm and 605nm. The ratio of OD570/OD605 is proportional to the number of cells shown.

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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