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Glucose Assay Kit (Fluorometric), High Sensitivity (CAT#: CB-P151-K) Datasheet

Product Type
Kit
Description
The Picoprobe Glucose assay Kit is a simple, fast and ultra-sensitive for the assay of glucose. Suitable for high throughput use. In this assay, D-glucose is oxidized by the enzyme to form a product, which reacts with a colorless probe to produce Fluorometric (Ex / Em = 535/587 nm). The Fluorometric produced is proportional to the amount of glucose. The test kit can detect glucose less than 0.5 µM in various biological samples.
Applications
Functional Studies
Size
1 kit
Detection Method
Fluorometric
Sensitivity
< 0.5 µM
Sample
Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells, Tissue Culture Media
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Glucose Assay Buffer 1 x 25ml
Glucose Enzyme Mix (lyophilized) 1 vial
Glucose Standard 1 x 100µl
Glucose Substrate Mix 1 vial
PicoProbe 1 x 0.4ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Glucose standard curve


Figure 2 Measurement of glucose levels in samples
Measurement of glucose levels in human serum (1 µL of 1:10 diluted) and rat tissue lysates from liver, kidney and muscle (0.14 µg, 0.19 µg and 0.93 µg respectively).

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All products and services are for Research Use Only. Do Not use in humans.

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