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Glycogen Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P149-K) Datasheet

Product Type
Kit
Description
The glycogen assay kit is a simple and accurate assay for measuring the glycogen content in biological samples. In the glycogen assay protocol, glucoamylase hydrolyzes glycogen into glucose, and then specifically oxidizes it to produce a product that reacts with the OxiRed probe to produce color (570 nm) and Fluorometric (Ex 535 / Em 587). This assay can detect glycogen at 0.0004 to 2mg/ml.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
0.4 µg/ml - 2000 µg/ml
Sensitivity
> 0.4 µg/ml
Sample
Tissue, Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Development Buffer 1 x 25ml
Development Enzyme Mix (lyophilized) 1 vial
Glycogen Standard (2.0 mg/ml) 1 x 100µl
Hydrolysis Buffer 1 x 25ml
Hydrolysis Enzyme Mix (lyophilized) 1 vial
OxiRed Probe 1 x 200µl
Requires
Microplate reader
Handling Advice
Glycogen assay protocol summary:
- add samples and standards to wells
- add hydrolysis enzyme mix and incubate for 30 min
- add reaction mix and incubate for 30 min
- analyze with microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Example of fluorometric standard curve using Glycogen Assay Kit.


Figure 2 Measurement of glycogen in various mouse tissues using Glycogen Assay Kit.


Figure 3 Glycogen concentration measured in MBA-MB-231 cells (human breast adenocarcinoma cell line). 106 cells were prepared following protocol instructions, and several dilutions were measured using fluorometric detection.

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All products and services are for Research Use Only. Do Not use in humans.

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