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L-Lactate Assay Kit (Colorimetric) (CAT#: CB-P140-K) Datasheet

Product Type
Kit
Description
The L-lactic acid assay kit (colorimetric) uses a measurement protocol in which lactic acid is oxidized by lactate dehydrogenase to produce a product that interacts with the probe to produce color (λmax = 450 nm).
The kit can detect L(+)-lactic acid in biological samples such as serum or plasma, cells, tissues, cell cultures and fermentation media.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Detection Limit
0.02 mM - 10 mM
Sensitivity
0.02 mM
Sample
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Species Reactivity
Mammals
Protocol Length
0h 30m
Components
L(+)-Lactate Standard (100 nmol/µl) 1 x 100µl
Lactate Assay Buffer 1 x 25ml
Lactate Enzyme Mix (lyophilized) 1 vial
Lactate Substrate Mix 1 vial
Requires
Microplate reader
Handling Advice
Lactate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Linearity of dilution: concentration of L-Lactate in differently diluted (X-axis) biological samples, demonstrating a linearity of 89%-111% (concentrations corrected for by factor of dilution; duplicates; +/- SD).


Figure 2 Relative signal (RFU) in unfiltered human plasma (dilution 1:8), comparing L-lactate signals with background reading (no enzyme) after 10 minutes of incubation (duplicates +/- SD).


Figure 3 Standard curve with background signal subtracted (duplicates; +/- SD).


Figure 4 Lactate Standard Curve.

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All products and services are for Research Use Only. Do Not use in humans.

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