Product Type
Antibody
Description
The monoclonal antibody M177 recognizes CD46, also known as membrane cofactor protein (MCP). CD46 is a 45-70 kDa protein with genetic and tissue-specific heterogeneity. -It is expressed in all cells and tissues except red blood cells. CD46 is used to inhibit complement activation on host tissues. It performs this function by acting as a cofactor that binds to C3b and C4b. Plasma serine protease factor I allows this combination to degrade C3b and C4b, and protects the host cell from autologous attack. It can also act as a receptor for measles virus. The four isoforms of CD46 are dominant and are produced by alternative splicing of a single CD46 gene. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). The monoclonal antibody M177 reacts with the SCR2 domain.
Disease
Infectious diseases, Tumor immunology
Size
100 µg
Immunogen
Purified MCP
Species
Human
Clonality
Monoclonal
Clone Number
M177
Conjugation
FITC
Buffer
PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
Application
Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno precipitation, Western blot
Application Notes
The diluent used depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50. For functional studies, the in vitro dilution must be optimized in the user's experimental setting.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Alternative Names
Membrane cofactor protein; TLX; trophoblast leucocyte common antigen
