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Amylase Assay Kit (Colorimetric) (CAT#: CB-P142-K) Datasheet

Product Type
Kit
Description
The amylase assay kit (colorimetric) detects the activity of α-amylase through a two-step reaction.


In the amylase assay protocol, α-amylase will cleave the substrate ethylene-pNP-G7 to produce smaller fragments, which are finally modified by α-glucosidase. This leads to the release of chromophores, which can then be measured at OD = 405 nm.


This assay can detect α-amylase content as low as 0.2 mU.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sensitivity
> 0.2 mU/well
Sample
Cell culture supernatant, Urine, Serum, Tissue Extracts, Cell culture media
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
mylase Assay Buffer 1 x 55ml
Amylase Positive Control1 vial
Amylase Substrate Mix1 x 5ml
Nitrophenol standard1 x 150µl
Requires
Microplate reader
Handling Advice
Amylase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader every 2-3 min for 30-60 min
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Standard curve: mean of duplicates (+/- SD) with background reads subtracted


Figure 2 Amylase activity measured in biological fluids showing activity (mU) per mL of tested sample. Samples were diluted 2 fold.


Figure 3 Amylase activity measured in tissue lysates showing activity (mU) per mg of extracted protein.


Figure 4 Sample Test

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All products and services are for Research Use Only. Do Not use in humans.

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