Description
PD-L1 (programmed cell death ligand 1; also known as CD274 or B7-H1) is a transmembrane protein expressed on the cell surface of hematopoietic and non-hematopoietic cells, induced by pro-inflammatory cytokines, such as in the tumor microenvironment in. PD-L1 is a ligand of PD-1, PD-1 is an inhibitory immune checkpoint receptor, expressed by activated and exhausted T cells. PD-1: PD-L1 interaction can induce the inhibition of T cell receptor signal transduction, thereby preventing T cell overstimulation and host damage.
Raji-hPD-L1 cells are developed from Raji cell line (a cell line derived from human B lymphocytes). Raji cells have been successfully used as target cells for CAR-T toxicity assays and human effect studies, such as antibody-dependent cytotoxicity (ADCC), which can be derived from peripheral blood mononuclear cells, natural killer (NK) cells or T cells Jurkat reporter cell.
Features
Surface expression markers and IC in Raji-hPD-L1 cells
Surface expression markers and IC in Raji-hPD-L1 cells
Stable overexpression of human PD-L1 gene
Characterized by many markers expressed on the cell surface, including B cell receptor (BCR), CD19 and CD20
Constitutive expression of various immune checkpoints (IC), such as CD27, CD70, CD80, PD-L1 and 4-1BBL
The stability of 20 channels after thawing has been verified
Applications
Used as a target cell line for ADCC analysis on Jurkat NFAT-CD16 cells
Used as target cell line NK or CAR-T cell for cytotoxicity determination
Used to develop other functional assays
Contents
Two kit formats are provided:
Complete, everything you need to get started
Core, used with customer-defined antibodies and target cells
Cell Background
Raji cell line
Sample
Works with both adherent and suspension cells.
Validation
Flow cytometry to verify PD-L1 overexpression
Used as a target cell for functional testing of anti-human PD-L1 monoclonal antibody and Jurkat NFAT-CD16 cells in ADCC analysis
Guaranteed Mycoplasma Free
Growth Medium
IMDM, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum (FBS; 30 min at 56 °C), 10 μg/ml Blasticidin, Pen-Strep (100 U/ml-100 µg/ml)
Test Medium
IMDM, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated FBS, Pen-Strep (100 U/ml-100 µg/ml)
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
Guaranteed mycoplasma-free
Storage
-80°C for short-term storage, liquid nitrogen for long-term storage
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
Our recombinant cell are for research use only, not for diagnostic or therapeutic use.
Quality Control
The expression of human PD-L1 has been verified by flow cytometry.
The induction of antibody-dependent cellular cytotoxicity (ADCC) has been verified using anti-hPD-L1-hIgG1 antibody and Jurkat-NFAT Lucia™ CD16 reporter cell line.
The stability of the 20 channels after thawing has been verified.
Raji-hPD-L1 cells are guaranteed to be free of mycoplasma.
