ADCC Control Target Cell Line (CD19, CD20, PD-L1), Human Raji Cells (CAT#: AD-P004-C) Datasheet

Description
The cell line was developed from the human Raji cell line. Raji cells constitutively express different antigens on their cell surface. These antigens can be targeted by specific antibodies, especially inducing antibody-dependent cellular cytotoxicity (ADCC).

-Human CD20 is highly expressed in Raji cells. It has no known natural ligands and is considered to be a calcium channel to achieve the best B cell response. In non-Hodgkin B-cell lymphoma or chronic lymphocytic leukemia with monoclonal antibody-mediated therapy (rituximab, ofatumumab, obinutuzumab), human CD20 is a clinically relevant target.

-Human CD19 is highly expressed by Raji cells. As a co-receptor, it lowers the threshold of antigen B cell receptor (BCR) dependent stimulation. Human CD19 is the clinical target of anti-hCD19-CD3 bispecific antibody (blinatumomab) in non-Hodgkin's B-cell lymphoma.

-Human PD-L1 (programmed cell death ligand 1; also known as CD274 or B7-H1) is expressed at low levels by Raji cells. This transmembrane protein is expressed in hematopoietic and non-hematopoietic cells and is induced by proinflammatory cytokines in the tumor microenvironment. Human PD-L1 is a clinically relevant target related to various cancers in mAb-mediated treatments (atezolizumab, durvalumab and avelumab).
Features
•Constitutive expression of CD19 and CD20 on cell surface
•Constitutive low expression of PD-L1 on the cell surface
•Stable expression of anti-elastin gene
Applications
•As ADCC target cells for anti-hCD19, anti-hCD20, and anti-hPD-L1 Abs
•As a negative control target cell in ADCC analysis of monoclonal antibodies other than anti-hCD19, anti-hCD20, and anti-hPD-L1 antibodies
•Target cells of anti-hCD19-CD3 bispecific antibody
Size
3-7 x 10e6 cells
Contents
Two kit formats are provided:
Complete, everything you need to get started
Core, used with customer-defined antibodies and target cells
Species Reactivity
Human
Cell Background
Raji cell line
Sample
Works with both adherent and suspension cells.
Validation
Used as a target cell for functional testing of anti-human CD20 monoclonal antibodies and Jurkat NFAT-CD16 cells in ADCC analysis
Use as a negative control target cells for monoclonal Abs other than anti-hCD20 (such as anti-hCTLA-4 or anti-hPD-1 Abs) for functional testing in ADCC analysis
Used as a target cell for functional testing of anti-hCD19-CD3 bispecific Ab and Jurkat NFAT cells in the T cell activation assay.
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
Guaranteed mycoplasma-free
Shipping
Dry ice
Storage
-80°C for short-term storage, liquid nitrogen for long-term storage
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
Our recombinant cell are for research use only, not for diagnostic or therapeutic use.
Quality Control
•Human CD20 expression has been verified by flow cytometry.
•The anti-hCD20-hIgG1 antibody and Jurkat-NFATCD16 reporter cell line have been used to verify the ability to induce antibody-dependent cellular cytotoxicity (ADCC).
•The stability of the 20 channels after thawing has been verified.
•Guaranteed to be free of mycoplasma.

Figure ADCC assay using various anti-human CD20 (Rituximab) antibody isotypes and Raji target cells

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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