ADCC NFAT-CD16 Effector Cell Line, Human Jurkat T Lymphocytes (CAT#: AD-P003-C) Datasheet

Description
NFAT-CD16 cells are engineered from the human T lymphocyte Jurkat cell line. Jurkat cells naturally express a functional NFAT (nuclear factor that activates T cells) transcription factor, which is involved in the early signaling event of antibody-dependent cellular cytotoxicity (ADCC). ADCC is an immune mechanism through which effector cells with Fc receptors can recognize and kill antibody (Ab)-coated target cells that express antigen on their surface. ADCC is triggered by cross-linking between Abs bound by immune effector cell surface antigens and the Fc receptor CD16A. These interactions induce an increase in intracellular calcium concentration, calreticulin/calmodulin-mediated dephosphorylation of NFAT, nuclear translocation and binding to the promoter region of ADCC-related genes. Eventually, the effector cells release cytotoxic particles that kill the target cells. Jurkat NFAT-CD16 cells have been designed as effector reporter cells for our ADCC analysis. These cells stably express the Lucia luciferase reporter gene under the control of ISG54 minimal promoter fused with six NFAT response elements. ADCC induction is measured by the bioluminescence signal generated by luciferase produced by adding the appropriate detection reagent Luc.
Features
•Stable expression of cell surface Fc receptor CD16A (FcgRIIIA; V158 high-affinity allotype)
•Stable expression of Lucia luciferase reporter gene under the control of ISG54 minimal promoter fused with six NFAT response elements
•Resistance to elastin
Applications
Measure Potency and Stability of Biobetter Antibodies that Bind and Activate ADCP through CD16
Size
3-7 x 10e6 cells
Contents
Two kit formats are provided:
Complete, everything you need to get started
Core, used with customer-defined antibodies and target cells
Species Reactivity
Human
Cell Background
Jurkat T Lymphocytes
Sample
Works with both adherent and suspension cells.
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
Guaranteed mycoplasma-free
Shipping
Dry ice
Storage
-80°C for short-term storage, liquid nitrogen for long-term storage
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
Our recombinant cell are for research use only, not for diagnostic or therapeutic use.
Quality Control
The expression of human CD16A has been verified by flow cytometry.
The induction of antibody-dependent cellular cytotoxicity (ADCC) has been verified using our anti-hCD20-hIgG1 antibody and Raji-Null target cell line.
The stability of the 20 channels after thawing has been verified.
Ensure mycoplasma-free.

Figure Jurkat-Luc NFAT cells induced by PMA+ionomycin, or by T-lymphocyte mitogen concanavalin A (ConA).

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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