ADCC NFAT Reporter (Luc) Effector Cell Line, Human Jurkat T Lymphocytes (CAT#: AD-P002-C) Datasheet

NFAT (Nuclear Factor that Activates T Cells) proteins are a family of transcription factors that are important in the development and function of the immune system. In T cells, NFAT protein regulates the development of thymocytes, T cell activation, differentiation and self-tolerance. Their activation is induced by binding to the T cell receptor (TCR) coupled to the calcium signaling pathway. Calcium binds calmodulin and activates calcineurin, which in turn dephosphorylates NFAT protein, thereby inducing its nuclear transport and activation and the transcription of specific response genes

NFAT-Luc Reporter T lymphocytes are derived from the Jurkat cell line based on human T lymphocytes by stably integrating the NFAT inducible Lucia reporter gene construct. The Lucia gene, which codes for the secretion of coelenterazine-using luciferase, is driven by the ISG54 minimal promoter fused with six copies of NFAT shared transcription response elements.

NFAT-Luc Reporter T lymphocytes respond to PMA and ionomycin and T lymphocyte mitogens, such as Concanavalin A (ConA) and phytohemagglutinin, to induce NFAT activation.

NFAT-Luc Reporter T lymphocytes can be used to stimulate NFAT activation studies after treatment by monitoring Lucia luciferase activity.

ADCC reporter effector cells