ADCC NFAT Reporter (Luc) Effector Cell Line, Human Jurkat T Lymphocytes (CAT#: AD-P002-C) Datasheet

Description
NFAT (Nuclear Factor that Activates T Cells) proteins are a family of transcription factors that are important in the development and function of the immune system. In T cells, NFAT protein regulates the development of thymocytes, T cell activation, differentiation and self-tolerance. Their activation is induced by binding to the T cell receptor (TCR) coupled to the calcium signaling pathway. Calcium binds calmodulin and activates calcineurin, which in turn dephosphorylates NFAT protein, thereby inducing its nuclear transport and activation and the transcription of specific response genes

NFAT-Luc Reporter T lymphocytes are derived from the Jurkat cell line based on human T lymphocytes by stably integrating the NFAT inducible Lucia reporter gene construct. The Lucia gene, which codes for the secretion of coelenterazine-using luciferase, is driven by the ISG54 minimal promoter fused with six copies of NFAT shared transcription response elements.

NFAT-Luc Reporter T lymphocytes respond to PMA and ionomycin and T lymphocyte mitogens, such as Concanavalin A (ConA) and phytohemagglutinin, to induce NFAT activation.

NFAT-Luc Reporter T lymphocytes can be used to stimulate NFAT activation studies after treatment by monitoring Lucia luciferase activity.
Applications
ADCC reporter effector cells
Size
3-7 x 10e6 cells
Contents
Two kit formats are provided:
Complete, everything you need to get started
Core, used with customer-defined antibodies and target cells
Species Reactivity
Human
Cell Background
Jurkat T Lymphocytes
Sample
Works with both adherent and suspension cells.
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
Guaranteed mycoplasma-free
Shipping
Dry ice
Storage
-80°C for short-term storage, liquid nitrogen for long-term storage
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
Our recombinant cell are for research use only, not for diagnostic or therapeutic use.
Quality Control
The expression of human CD16A has been verified by flow cytometry.
The induction of antibody-dependent cellular cytotoxicity (ADCC) has been verified using our anti-hCD20-hIgG1 antibody and Raji-Null target cell line.
The stability of the 20 channels after thawing has been verified.
Ensure mycoplasma-free.

Figure Jurkat-Luc NFAT cells induced by PMA+ionomycin, or by T-lymphocyte mitogen concanavalin A (ConA).

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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